pcdna3 ha sam68wt vector Search Results


xhoi  (New England Biolabs)
99
New England Biolabs xhoi
Xhoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/xhoi/product/New England Biolabs
Average 99 stars, based on 1 article reviews
xhoi - by Bioz Stars, 2026-03
99/100 stars
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pcdna3 ha sam68 wt vector  (Addgene inc)
93
Addgene inc pcdna3 ha sam68 wt vector
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Pcdna3 Ha Sam68 Wt Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 ha sam68 wt vector/product/Addgene inc
Average 93 stars, based on 1 article reviews
pcdna3 ha sam68 wt vector - by Bioz Stars, 2026-03
93/100 stars
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bamhi  (New England Biolabs)
99
New England Biolabs bamhi
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bamhi/product/New England Biolabs
Average 99 stars, based on 1 article reviews
bamhi - by Bioz Stars, 2026-03
99/100 stars
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halotag7 insert  (Addgene inc)
91
Addgene inc halotag7 insert
a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and <t>HaloTag7-Sam68.</t> e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.
Halotag7 Insert, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/halotag7 insert/product/Addgene inc
Average 91 stars, based on 1 article reviews
halotag7 insert - by Bioz Stars, 2026-03
91/100 stars
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Image Search Results


a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

Journal: bioRxiv

Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

doi: 10.1101/2022.10.24.513449

Figure Lengend Snippet: a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

Article Snippet: The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates.

Techniques: Construct, Expressing, Incubation, Imaging, Fluorescence, Plasmid Preparation

a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

Journal: bioRxiv

Article Title: Fast-exchanging spirocyclic rhodamine probes for aptamer-based super-resolution RNA imaging

doi: 10.1101/2022.10.24.513449

Figure Lengend Snippet: a) Schematic illustration of the construct used for expressing RhoBAST-tagged G FP mRNA. b) Confocal images of HEK293T cells expressing GFP-RhoBAST 16 or GFP (control) mRNA incubated with SpyRho (100 nM) for 1 h before imaging. c) Normalized fluorescence profile along the dashed line in panel b (lower le). d) Schematic illustration of the constructs used for expressing CGG-containing FMR1-GFP mRNAs fused to 16 repeats of RhoBAST and HaloTag7-Sam68. e) Confocal images of live Cos7 cells expressing CGG99-FMR1-GFP-RhoBAST 16, CGG99-FMR1-GFP or GFP (control) mRNA and HaloTag7-Sam68 fusion protein (plasmid ratio 10:1) incubated with SpyRho (100 nM) and MaP700-Halo (200 nM, SiR channel) for 1 h before imaging. f) Normalized fluorescence profiles along the dashed lines shown in e). The fluorescence in the TMR channel was normalized to the highest value detected for CGG99-FMR1-GFP mRNA. Scale bars, 5 μm.

Article Snippet: The vector backbone and the HaloTag7 insert were amplified by PCR using the pcDNA3-HA-Sam68-WT vector (Addgene, plasmid #17690) and pcDNA5-FRT-Halo-SNAP-NLS plasmid (kindly provided by the Johnsson group, Heidelberg) as templates.

Techniques: Construct, Expressing, Control, Incubation, Imaging, Fluorescence, Plasmid Preparation